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| Registros recuperados: 14 | |
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| Claveria, Florencia G.; Omata, Yoshitaka; Takehara, Tokuma; Saito, Atsushi; Maki, Yoshiyuki; Igarashi, Ikuo; Suzuki, Naoyoshi. |
| Anti-Babesia rodhaini monoclonal antibodies (mAb), namely: 1-E7, 2-H2 and 3-B8, significantly suppressed the development of high parasitemia in BALB/c mice infected with B. rodhaini and all mAb-treated mice survived the infection. While, only monoclonal antibody 3-B8 showed some inhibitory effect against Babesia microti, with mice showing high parasitemia of 18.04 ± 2.69 %, at 9 days post-exposure. Westernblot analysis of B. rodhaini and B. microti parasite extract reacted with anti-B. rodhaini monoclonal antibodies showed cross-reactive bands of molecular weights 62 and 55 kilodaltons. Comparison of the antigenic components of B. rodhaini and B. microti using polyspecific sera revealed several shared or common parasite antigens of molecular weights 62,... |
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Palavras-chave: Monoclonal antibodies; Cross-reactivity; Babesia rodhaini; Babesia microti. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/171 |
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| Hara, Olgga A; Liao, Min; Baticados, Waren; Bannai, Hiroshi; Zhang, Guohong; Zhang, Sofa; Lee, Eung-goo; Nishikawa, Yoshifumi; Claveria, Florencia G.; Igarashi, Makoto; Nagasawa, Hideyuki; Xuan, Xuenan. |
| Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were... |
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Palavras-chave: Canine; ELISA; Neospora caninum; NcGRA7. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/995 |
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| Ybañez, Adrian Patalinghug; Ybañez, Rochelle Haidee D.; Claveria, Florencia G.; Cruz-Flores, Mary Jane; Xuenan, Xuen; Yokoyama, Naoaki; Inokuma, Hisashi. |
| A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginale infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein 1a (Msp1a) gene-PCR assay. Screening PCR results showed 130 cattle (19.8%) were positive for the A. marginale infection. Subsequent amplification using the Msp1a gene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeat structures, including 20 novel structures, and 41 distinct genotypes were identified. Interestingly, multiple infections of 4 different genotypes were also observed in A. marginale-infected cattle. The present study demonstrated the prevalence and... |
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Palavras-chave: Anaplasma marginale; Cattle; GroEL; Msp1a; Philippines. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4011 |
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| Santiago, A. T. A.; Claveria, Florencia G.. |
| Surveillance of the presence of larval mosquitoes was performed in the suburban municipality of Sta. Ignacia, Tarlac Province, Philippines, with approximately 94.0% of the land area still devoted to agriculture. Five districts were covered, namely: Macageuing, San Francisco, San Vicente, Sta. Ines Centro, and Sta. Ines East. The standard dipping method was used to collect mosquito larvae from rice fields, ponds, creeks, streams and used tires and larvae were identified at the genus level. Culex sp. was the most abundant with a calculated mean breeding index of 22 larvae per dip. Anopheles sp. and Aedes sp. were likewise identified with mean breeding indices of five and two larvae per dip, respectively. In two samplings, Culex sp. had the highest mean... |
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Palavras-chave: Mosquito larval surveillance; Breeding index; Sta. Ignacia; Tarlac Province; Philippines. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1834 |
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| Salibay, C. C.; Dungca, J. Z.; Claveria, Florencia G.. |
| Serological survey of Toxoplasma gondii infection was carried out in 68 urban (Manila) and 72 suburban (Dasmariñas, Cavite) residents, comprising 69 females and 71 males, age 16-56 years old. Using a survey questionnaire, the name and address, gender, age, weight, height, civil status, cat exposure, and additional information like history of pregnancy and current reproductive status of females were obtained. The presence of anti-T.gondii antibodies was confirmed using the Toxocell latex agglutination test. The pooled population revealed 38 (27.1%) seropositive (sero+) cases, with higher infection rate among the suburban (30.6%) and female (32.0%) respondents. Differences in percent infection between the study groups, sexes and respondents with normal,... |
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Palavras-chave: Serological assay; Toxoplasma gondii; Urban and suburban dwellers; Philippines. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1835 |
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| Kato, Mihoko; Maki, Yoshiyuki; Inoue, Noboru; Omata, Yoshitaka; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男. |
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Palavras-chave: Cytotoxic cell activity; NK cell; LAK cell; Toxoplasma lysate antigen. |
| Ano: 1994 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/216 |
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| Registros recuperados: 14 | |
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